Biomedical imaging

Blood vessel imaging without contrast agents
Micrometastasis imaging without contrast agents
Angiogenesis (within metastatic tumors) imaging without contrast agents


Blood vessel imaging without contrast agents

        In collaboration with the researchers at Med-x research institute, Shanghai Jiao Tong University, we have successfully imaged the carotid artery and the carotid vein in a formalin-fixed mouse in-situ without contrast agents, paving the way for future applications in cancer angiography studies. The work has been published on J. Synchrotron. Rad. 19, 821, (2012).  ( PDF )

Figure:The bood vessels in mouse chest. (a) The reconstructed slice of the mouse chest by GPC-CT, (b) Enlarged view of the blue box in (c), which reveals the presence of three blood vessels. Scale bar: 2mm.




Micrometastasis imaging without contrast agents

        In collaboration with Shanghai Cancer Center of Fudan University, we have compared the efficiencies of IPC-CT (inline phase-contrast CT) and GPC-CT at a synchrotron X-ray source for the detection of micro-metastases in a brain metastasis model of human breast cancer. We found that both IPC-CT and GPC-CT can differentiate abnormal brain structures induced by micro-metastases from the surrounding normal tissues. Furthermore, GPC-CT was found to be more sensitive in detecting the small metastases as compared to IPC-CT. The work has been published on Scientific Reports, 5, 9418 (2015).  ( PDF )

Figure:The hippocampus metastasis in brain sample. (a) H&E pathological graphs,(b) and GPC-CT slice. Scale bar: 0.2mm




Angiogenesis (within metastatic tumors) imaging without contrast agents

        In collaboration with Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, we established lung metastases models by injecting human moderately differentiated gastric cancer SGC-7901 cell line into the tail vein of nude mice and imaged the angiogenesis within metastatic tumors without contrast agents. Vessels, down to tens of microns, showed gray values that were distinctive from those of the surrounding tumors and were similar to those identified on histopathology, both in size and shape. This work demonstrates that GPC-CT has the potential to depict the angiogenesis of lung metastasis. The work has been published on PLoS one. 10, e0121438, (2015).  ( PDF )

Figure: The branch-like blood vessels with blood cells inside within the tumor in lung metastasis sample. (a) H&E pathological graphs and (b) GPC-CT. Scale bar: 0.5mm.